ANTI-ARTHRITIC AND ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC LEAF EXTRACT OF ORMOCARPUM SENNOIDES

摘要

Objective: The study focuses on evaluating the anti-inflammatory and anti-arthritic activity of ethanolic extract of Ormocarpum sennoides (Os), a herb belonging to the family fabaceae. Both in vitro and in vivo methods were employed to study the anti-inflammatory activity of Os extract. Methods: The in vitro analysis of Os extract was performed by HRBC membrane stabilization and protein denaturation methods. The in vivo analysis was done by Formalin-induced paw edema and acetic acid induced writhing test using wistar male albino rats as an animal model. Results: The in vitro analysis revealed the inhibition of hemolysis and protein denaturation in a dose-dependent manner in concentrations ranging from 50-500 μg/ml. The in vivo study showed that Os extract reduced the number of paw lickings and abdominal writhing significantly at dosage concentrations of 200 mg/kg body weight and 300 mg/kg body weight compared with standard diclofenac sodium (p<0.05). Conclusion: These results support the application of Os extract in ailing arthritis and treatment of painful inflammatory conditions. Keywords: Anti-inflammatory activity, Anti-arthritic activity, Ormocarpum sennoides , Rheumatoid arthritis