STUDES ON ANTIBACTERIAL and ANTICANCER ACTIVITY of Nerium oleander EXTRACTS

摘要

Isolation had been done for active substances from Nerium oleander plant ( Nerium oleander ) such as volatile oils and Tannins. The percentage in form was (11.1%),(39.2%) respectively. As well as the appointment of some mineral elements in the Nerium oleander seeds like sodium, calcium and potassium and the concentration in the sample was (107)ppm, (96) ppm, (73) ppm, respectively, measured by using Flame Spectrometer. Also a study of anti-bacterial activity of extracts from Nerium oleander using two types of pathogenic bacteria Escherichia Coli and aurous Staphylococcus showed the ability of inhibition for all different extracts by vary inhibition diameters for different active substances, concentrations and bacteria. One type of cancer cellular line used to study the effect of Nerium oleander extracts (after the chemical assay) on the growth of (L 20 B) cell line by using in vitro system and compared with anticancer drug cisplatin (cis-pt) as a positive control. The cancer cell were treated with different concentration for each of the three treatments and cis-pt after 72hr. exposure time. The cytotoxic activity was tested by inhibition rate as parameter . the results showed significant differences (p<0.05) for each three treatments when the inhibition rates were increased. There was strong correlation between the three treatments and the different concentrations in comparison with cisplatin.