Genetic similarity and antimicrobial susceptibility of Klebsiella pneumoniae producing carbapenemase (KPC-2) isolated in different clinical specimens received from University Hospitals in Northeastern Poland

摘要

Worldwide scientific data show that there is an increasing level of resistance to carbapenems among?Enterobacteriaceae?rods. We examined the genetic similarity and antimicrobial susceptibility of four KPC-2-positive?Klebsiella pneumoniae?(with geneblaKPC)?which were isolated (for the first time) in two University Hospitals in northeastern Poland. Isolates were obtained from March 2011 to May 2011. The minimum inhibitory concentrations (MICs) of thirteen antibiotics were determined for each isolate. Three of?K. pneumoniae?isolates (USK1/1, USK2/1 and USK4/1) were intermediate to imipenem (MICs 4 mg/L), and only one isolate (DSK1/1) was resistant to imipenem (MIC >32 mg/L). In addition they were intermediate or susceptible to meropenem (MICs 1-8 mg/L) and doripenem (MICs 2-4 mg/L). Genotyping and dendrogram analysis of four KPC-producing isolates were performed using the repetitive sequence-based polymerase chain reaction (rep-PCR) in the DiversiLabTM?Microbial Typing System. Two rep-PCR clusters (A and B) were determined in University Hospitals in the same city (Bialystok, Poland). Among KPC-2-producing isolates, three isolates (USK1/1, USK2/1, USK4/1) with a similarity of >98% belonged to cluster A, and one (DSK1/1) was dissimilar (<95%) to all the other isolates.