Bone marrow mesenchymal stem cell from chronic hepatitis B patients differentiation into hepatocyte-like cells

摘要

The aim of this study was to observe the biological characteristics and hepatic differentiation potential of bone marrow mesenchymal stem cells (BM-MSCs) from chronic hepatitis B patients?in vitro. MSCs were isolated from bone marrow of 15 chronic hepatitis B patients and 11 normal donors by density gradient centrifugation. The adipogenic and osteogenic differentiation potential, immunophenotyping and DNA contents were detected. Chronic hepatitis B patients BM-MSCs were induced to hepatocytes. Hepatic markers were detected by immunohistochemical analysis. Periodic acideschiff staining was used to detect for glycogen production. The results showed that both MSCs had adipogenic and osteogenic differentiation potential, nearly all the BM-MSCs expressed CD105, CD166, CD44 and CD29. Compared with normal donors, the primary passage time of BM-MSCs from chronic hepatitis B patients were much longer, and the S-phase nuclei were lower. Chronic hepatitis B patients BM-MSCs were induced to hepatocyte-like cell, which express the hepatic-specific markers and have the glycogen production function. Though it was difficult to cultivate BM-MSCs from chronic hepatitis patients?in vitro?and they had deficient proliferation, they could differentiate into functional hepatocyte-like cells.