Development of PCR method for fast detection of Ophiostoma floccosum in wood chips

摘要

Ophiostoma?floccosum?is one of blue stain fungi that cause cosmetic damage in softwood. To develop a molecular maker for the detection of?O. floccosum, we designed Oflo1 and Oflo2 primers based on the β-tubulin gene. PCR assay with eleven isolates ofO. floccosum?and nine other blue stain and discoloring fungal species (moulds) proved that the designed primers specifically amplified a 333 bp sized DNA band only from genomic DNA of?O. floccosum?isolates. Together with microwave heating extraction of spore DNA, we could?detect?O. floccosum?within 2 h on artificially inoculated wood chip. We believed that the developed PCR method will be useful where detection of target species such as?O. floccosum?is required.