A Model and Analysis of the AKAP Scaffold

摘要

We study the biochemical processes involved in scaffold-mediated crosstalk between thecAMPand theRaf-1/MEK/ERKpathways. We model the system by a continuous time Markov chain with levels and analyse properties using Continuous Stochastic Logic and the symbolic probabilistic model checkerPRISM. We consider two kinds of properties of the model, causal events and pulsating behaviour, and, in order to formulate these properties, we enrich the model with trend formulas. The system is currently under wet-lab investigation and our approach was developed in collaboration with the experimentalists.