Transcriptome and proteome analyses of resistant preharvest peanut seed coat in response to Aspergillus flavus infection

摘要

BackgroundThe infection of peanut (Arachis hypogaeaL.) seed coat by the pathogenic fungusAspergillus flavushas highly negative economic and health impacts. However, the molecular mechanism underlying such defense response remains poorly understood. This study aims to address this issue by profiling the transcriptomic and proteomic changes that occur during the infection of the resistant peanut cultivar J11 byA. flavus.ResultsTranscriptomic study led to the detection of 13,539 genes, among which 663 exhibited differential expression. Further functional analysis found the differentially expressed genes to encode a wide range of pathogenesis- and/or defense-related proteins such as transcription factors, pathogenesis-related proteins, and chitinases. Changes in the expression patterns of these genes might contribute to peanut resistance toA. flavus. On the other hand, the proteomic profiling showed that 314 of the 1382 detected protein candidates were aberrantly expressed as a result ofA. flavusinvasion. However, the correlation between the transcriptomic and proteomic data was poor. We further demonstrated by in vitro fungistasis tests thathevamine-A, which was enriched at both transcript and protein levels, could directly inhibit the growth ofA. flavus.ConclusionsThe results demonstrate the power of complementary transcriptomic and proteomic analyses in the study of pathogen defense and resistance in plants and the chitinase could play an important role in the defense response of peanut toA. flavus. The current study also constitutes the first step toward building an integrated omics data platform for the development ofAspergillus-resistant peanut cultivars.How to cite:Zhao X, Li C, Yan C, et al. Transcriptome and proteome analyses of resistant preharvest peanut seed-coat in response toAspergillus flavusinfection. Electron J Biotechnol 2019;39.https://doi.org/10.1016/j.ejbt.2019.03.003.