EFFECT OF OOCYTE ACTIVATION WITH CALCIUM IONOPHORE ON ICSI OUTCOMES IN TERATOSPERMIA: A RANDOMIZED CLINICAL TRIAL

摘要

Background: Chemical activation is the most frequently used method for artificial oocyte activation (AOA), results in high fertilization rates.Objective: This prospective, randomized, unblinded clinical study aimed to evaluate the efficiency of calcium ionophore on fertilization and pregnancy rate after intracytoplasmic sperm injection (ICSI) in infertile men suffer from teratoospermia.Materials and Methods: Thirty eight women with teratoospermic partner underwent ICSI with GnRH-antagonist protocol. A total of 313 metaphase II (MII) oocytes were randomly divided after ICSI into two groups: In the oocytes of the control group (n=145), routine ICSI was applied. Oocytes in the AOA group (n=168) immediately after ICSI, were cultured in culture medium supplemented with 5