PROTECTIVE EFFECTS OF L-CARNITINE AND HOMOGENIZED TESTIS TISSUE ON THE TESTIS AND SPERM PARAMETERS OF BUSULFAN-INDUCED INFERTILE MALE RATS

摘要

Background: Busulfan (Bus) is a chemotherapy drug that is widely used for cancer treatment. However, administration of busulfan may cause temporary or permanent sterility in male patients. Therefore, reduction of this side is necessary.Objective: evaluation of the protective effects of L-carnitine and testis homogenized tissue (THT) on sperm parameters and the testis structure after busulfan treatment.Materials and Methods: Twenty rats were divided four groups. Group I (Control) received a single dose of DMSO and 1mL of distilled water (I.P.). Group II (Bus) received a single of busulfan (10 mg/kg) plus 1 ml of the distilled water (I.P.). Group III (Bus+THT) received busulfan plus 1mL of THT daily by oral gavages. Group IV (Bus+L-car) received a single dose of busulfan plus 100 mg/kg/day L-carnitine (I.P.). After 48 dayst, the Stereological technique was used for the estimating volume and diameter of testis, seminiferous tubules and interstitial tissue, flagella length, germinal epithelium height and spermatoginic cell number. Semen analysis was used for the assessment of sperm parameters.Results: THT increased volume of testis (6.5%), seminiferous tubule and interstitial tissue volume (6.5%, 6.9% and 11.7% respectively), germinal epithelium height (13%), sperm count (7.5%), and decreased sperm with abnormal morphology (1%) in comparison with the L-carnitine in busulfan treated group.Conclusion: It seems the use of L-carnitine and THT decreases side effects of busulfan on the male reproductive system. However, in our study, THT is more effective than L-carnitine and leads to the recovery testis structure and sperm parameters after treatment with busulfan.