EFFECTS OF MELATONIN ON HISTOPATHOLOGICAL CHANGES AFTER EXPERIMENTAL OVARIAN TORSION-DETORSION IN CAT

摘要

Background: During the detorsion of a torsioned ovary, oxidant agents are released and melatonin as an antioxidant can reduce ischemia. We studied the histopathological changes after using melatonin on experimental torsioned ovary in cat.Objective: The aim of this experimental study was to investigate the effects of Melatonin on histopathological changes in torsion a€“ detorsion injury in cat ovary.Materials and Methods: An adnexal torsion a€“ detorsion model was created by using 20 adult cats randomly divided equally in to 2 groups of Saline and Melatonin. Ischemia was induced by iathrogenic 360?° clockwise torsion of the cat adnex for 3 hr. Reperfusion was achieved for 3 hr. Melatonin or saline were injected intra peritoneally (10mg/kg) 30 min before ovarian detorsion in both groups. After 3 hr of ovarian detorsion, ovarian tissue was removed and fixed in 10% formalin solution, embedded in paraffin and evaluated for ischemic indices.Results: Histological examination showed a significant improvement in ovarian morphology in the melatonin treated cats. Edema and vasoconstriction in saline group were more severe than Melatonin group (p-value = 0.009). Hemorrhage and leukocyte infiltration were also more obvious in saline group (p-value 0.0018).Conclusion: Our results demonstrated that Melatonin administration reduced ovarian histopathological damage due to oxidative injury associated with torsion.