Evaluation of effectiveness of an innovative semen extender (Formula?) comparing with a traditional extender (Lepus?) for artificial insemination in rabbits does

摘要

This study aimed to investigate the preservability and viability of the rabbit spermatozoa diluted in a new semen extender Formula? in comparison with Lepus? at 17?°C of storage. The main characteristic of the new extender formulation is the use of an enzymatic agent associated to a polysaccharide as energy source precursor, added with gentamycin. During eight trials, ejaculates from 70 bucks were collected and diluted at 1:10 ratio with both the extenders, after 24?h of storage the semen doses were used for the artificial insemination (AI). Aliquots of the semen doses for each trial were stored at 17?°C, the total and progressive motility were checked at 0, 4, 12, 18, 24, 36, 48, 60, 72, 84, 96, 108?h of storage. A total of 1267 and 1525 does were inseminated, respectively with Formula? and Lepus?. During storage the mean total and progressive motility (77.23% and 72.854%, respectively) were significantly higher for Formula? (p?? with significant differences after 12?h of storage (p?p?p?? vs 8.51 with Lepus? (p?? is recommended for rabbit semen AI programmes.