Expression profiles of novel genes and microRNAs involved in lipid deposition in chicken’s adipocyte

摘要

HuaYun Huang ab , GuiPing Zhao a , RanRan Liu a , ShouFeng Li b , ZhenHua Zhao b , QingHe Li a , MaiQing Zheng a & Jie Wen a* a Institute of Animal Sciences , Chinese Academy of Agricultural Sciences , Beijing , P.R. China ; b Institute of Poultry Science , Chinese Academy of Agriculture Sciences , Yangzhou , P. R. China Supplemental data for this article can be accessed here . CONTACT Professor Jie Wen wenjie@caas. cn Institute of Animal Sciences , Chinese Academy of Agricultural Sciences , Beijing 100193 , P.R. China With the aim of studying the molecular mechanisms underlying lipid deposition in chickens, the expression profiles of eight novel candidate genes and miRNA involved in lipid deposition were examined in the undifferentiated and differentiated adipocyte at 3d, 5d and 10d after inducing differentiation. The level of expression of gga-miR-30 cluster (gga-miR-30a-5p, miR-30c-5p and miR-30e-5p) and miR-17-92 cluster (gga-miR-17-5p, miR-19a-3b and miR-20a-5p), compared to undifferentiated adipocyte, was significantly up-regulated with differentiated adipocyte; expression of miR-103-3p and miR-92-5p was significantly up-regulated with differentiated adipocyte. Seven genes, ACSL1, CYB5A, SEC23A, BRP44L, PLTP, MGLL and GART, compared to undifferentiated adipocyte, were significantly down-regulated with differentiated adipocyte. Correlation analysis of the detected genes and miRNAs indicated BRP44L may be a target of miR-103-3p and it was negatively correlated with miR-103-3p. CYB5A may be a target of miR-30a-5p and it was negatively correlated with miR-30a-5p. SEC23A may be a target of miR-19a-3p and it was negatively correlated with miR-19a-3p. MGLL may be a target of miR-19a-3p and it was negatively correlated with miR-19a-3p. These findings will improve more integrated information of the miRNA and genes in regulating the quantity of lipid deposition in chicken adipocyte.