MicroRNA Expression Profile in Insulin-Resistant 3T3-L1 Adipocyte

摘要

MicroRNAs (miRNAs) are a class of small non-coding RNAs which play an important role in metabolism of glucose and lipid in adipocyte. This study investigated miRNAs expression profiles during the formation process of insulin-resistant adipocyte derived from 3T3-L1 adipocyte. miRNAs were examined by miRNAs microarray analysis..The results showed that 71 miRNAs were upregulated and 33 miRNAs were downregulated during the formation of insulin resistance. Among them, miRNA (miR)-320, -494, -503, -202, -185 and -129-5p were significantly increased. Western blotting analysis showed that Anti-miR-320 increased GLUT4 expression.