Genetic Variability of Calpastatin and Calpain Genes in Iranian Zel Sheep Using PCR-RFLP and PCR-SSCP Methods

摘要

The genotypes for calpastatin (CAST) and calpain (CAPN) loci were determined by PCR-RFLP and PCRSSCPmethods. Blood samples were collected from 200 pure-bred Zel sheep from Shirang’s Zel sheep Breeding Station located in south-west of Golestan, Iran. Extraction of genomic DNA was performed based on the modified salting out method. Based on results, two investigated loci were polymorphic and had differentgene variants. Heterozygosis was low for both loci. Chi-square test confirmed Hardy-Weinberg equilibriumonly in CAST locus using SSCP method. Detected polymorphisms and associations of genetic variationwith meat production and tenderness may help to find the effective genotypes of Zel sheep for the economictraits.