The present invention relates to the rpoB DNA for particularly amplifying mycobacterium tuberculosis for being used to detect rifampin-resistant Mycobacterium tuberculosis by nested PCR single strand conformation polymorphism the present invention relates to a kind of method to amplify another bacterium and the first PCR using a priming paint of the main PCR of the rpoB DNA of the first PCR without using the priming paint of the rpoB DNA for amplifying 157 bp, it executes a grade PCR using a priming paint for secondary PCR, it is used to amplify the nested PCR single strand conformation polymorphism for shortening the rpoB DNA and a single-stage that execute program. Electric primer is used in the case where NPCR-SSCP, since the high specificity of priming paint is the first PCR, it only can particularly amplify the DNA of mycobacterium tuberculosis, therefore the false positives as caused by non-tuberculous acidophilic bacteria (MOTT) are prevented, it can handle a large amount of sample, therefore greatly save time and spend.
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