Antiangiogenic Activity of Tinospora rumphii Boerl (Makabuhay) Leaf and Stem Extracts

摘要

In the Philippines, many herbal plants are being used traditionally for the treatment of common diseases and disorders. There are medicinal plants which inhibit angiogenesis or the formation of new blood vessels. One of them is the Tinospora rumphii Boerl, a large, abundantly growing vine, commonly known as “Makabuhay”. It is being used by rural folks for the treatment of cancerous wounds and tropical ulcers. This study evaluated the antiangiogenic activity of T. rumphii leaf and stem extracts using the number of new blood vessels formed in the chorioallantoic membrane of fertilized mallard embryos after 48 hours of treatment. Results of the CAM assay showed that the T. rumphii extracts exhibit antiangiogenic property based on the less blood vessels formed in the chorioallantoic membrane of mallard embryos. The combination of T. rumphii leaf and stem extracts yielded the lowest mean number of new blood vessels formed as compared to the leaf extract and the stem extract. One-way ANOVA revealed that there is a significant difference in the mean number of blood vessels formed, while method of Least Significant Difference specified that the combination of T. rumphii leaf and stem extracts is significantly more antiangiogenic than the leaf extract and the stem extract. These findings led to the conclusion that the T. rumphii extracts have antiangiogenic potential and that the combination of leaf and stem extracts inhibit angiogenesis more than the leaf extract or the stem extract.