Detection and Molecular Characterization of Infectious Bronchitis virus Isolated from Recent Outbreaks in Chicken Farms in Egypt 2012

摘要

Egyptian viruses of infectious bronchitis of 13 isolates were collected between period from 1/1/2012 to 1/8/2012 from 200 samples were collected and examined with PT-PCR then choose positive sample from different governorates. Thirteen avian Infectious Bronchitis virus (IBV) isolates were isolated from broiler chickens showing respiratory signs. The isolated strains were characterized by reverse transcriptase polymerase chain reaction and sequence analysis of the hypervariable region of the S1 spike glycoprotein gene and study different and identity between them then compare between them by using phylogenetic analysis using Megalign software. Eleven out of thirteen isolates formed adistinct phylogenetic group with (IS/885) with identity 89.9%. Two of the thirteen isolates showed 97% amino acid sequence identity between them and 82.3% nucleotide sequence identity to the Egyptian variant 2 and the IS/1494/06 strains, respectively.