首页> 中文期刊>东北农业大学学报 >鸡传染性支气管炎病毒ck/CH/LHB/121042毒株的分离鉴定及分子特征研究

鸡传染性支气管炎病毒ck/CH/LHB/121042毒株的分离鉴定及分子特征研究

     

摘要

鸡传染性支气管炎病毒(infectious bronchitis virus,IBV),严重威胁养禽业健康发展.近年来,我国不断出现新型IBV毒株及变异株,给传染性支气管炎(infectious bronchitis,IB)防控造成困难.监测研究报告显示,IBV感染与多种类型毒株有关,主要以LX4型为主.我国部分地区近期出现LX4型与TW型重组毒株,但鲜有TWⅡ毒株报道,其分子特征和抗原特性目前尚不清楚.通过分离河北省某鸡场疑似IB病鸡肾脏,获得传染性支气管炎病毒ck/CH/LHB/121042,基因组全长27 675bp,共10个开放阅读框,基因组特征为5'UTR-1a-1b-S-3a-3b-3c-M-5a-5b-N-3'UTR,Spike protein裂解酶识别位点为“Arg-Arg-Phe-Arg-Arg”.病毒基因型鉴定为TWⅡ型,基因组遗传进化分析表明,该毒株与2010年河北分离株ck/CH/LHB/100801亲缘关系最近,推测具有相同进化来源.研究结果对于阐明病毒起源、预测未来产生新型毒株和重组毒株等具有重要意义.%Infectious bronchitis virus (IBV),caused considerable damage to poultry industry,exists in a wide range of the world.In recent years,new genotypes of IBV and associated variants appeared frequently in different parts of China,resulting in a major obstacle for the prevention and control of this pathogen.Surveillance studies have reported that widespreadly IBV infection in China is related to multiple types.Of these,LX4 (QX-like) is the predominant type circulating in chicken flock.However,in a recent study,recombinant IBV originated from QX-type and TW-type strains was reported in some parts of China.Up to now,a few reports on TW Ⅱ strains is officially demonstrated in China.As the result,the molecular and antigenic characteristics of lWⅡstrains were not clear.In this study,an avian infectious bronchitis virus,designated as cklCH/LHB/121042,was isolated and identified from kidney of chickens,which was suspected to be infected with IBV in Hebei Province,China.The result showed that ck/CH/LHB/121042 strain was 27 675 bp,with 10 ORFs,characterized by the 5'-cas-Replicase-S-3a-3b-3c-M-5a-5b-N-poly(A)-3'gene order,and the typical avian coronavirus cleavage site of spike protein contained five consecutive basic amino acids,namely,Arg-Arg-Phe-Arg-Arg.Based upon data arising from S1 sequence and phylogenetic analysis,the isolate was TW Ⅱ genotype and closely related to ck/CH/LHB/100801 strain.Based on this finding,it was speculated that this IBV might derive from ck/CH/LHB/100801 strain.Retrospective studies,involving molecular characterization,are necessary to clarify the origin of the viruses and are significant for the prediction of new variants and recombinants.

著录项

  • 来源
    《东北农业大学学报》|2018年第2期|55-63|共9页
  • 作者单位

    东北农业大学动物科学技术学院,哈尔滨150030;

    东北农业大学动物科学技术学院,哈尔滨150030;

    中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室禽传染病研究室,哈尔滨150001;

    东北农业大学动物科学技术学院,哈尔滨150030;

    中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室禽传染病研究室,哈尔滨150001;

    东北农业大学动物科学技术学院,哈尔滨150030;

    中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室禽传染病研究室,哈尔滨150001;

    中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室禽传染病研究室,哈尔滨150001;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 稻;
  • 关键词

    鸡传染性支气管炎病毒; 分离; 全基因组序列;

  • 入库时间 2023-07-25 16:12:41

相似文献

  • 中文文献
  • 外文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号