Gene Expression Pattern of Insulin-Like Growth Factor-I Receptor on Mesenchymal Stem Cells Induced by Tumor Necrosis Factor-a

摘要

Cell-based therapy has been implicated in treatment in a wide range of diseases. Mesenchymal stem cells from various sources such as bone marrow are available. These cells are one of the major candidates in cell therapy. The production of insulin-like growth factor-I increases in the regenerating organ. The insulin-like growth factor-I in liver regeneration is effective after binding to insulin-like growth factor-I receptor. We hypothesized that tumor necrosis factor- stimulate mesenchymal stem cells to cause insulin-like growth factor-I receptor expression. Bone marrow was aspirated from human normal donor after informing consent. Cells were isolated and cultured. Identification of cells with flow cytometric analysis and functional tests were performed. Fourth passage cells were treated with tumor necrosis factor- at different doses (1 ng/mL and 10 ng/mL) and incubated at different times (2, 10, 24 and 48 hours). Insulin-like growth factor-I receptor gene expression was investigated using real time-polymerase chain reaction technique. Flow cytometric analysis showed that the human bone marrow mesenchymal stem cells were positive for CD90 and negative for CD45 and CD80. The insulin-like growth factor-I receptor gene expression was increased in tumor necrosis factor- treated in comparison with untreated cells. Increase gene expression pattern of insulin-like growth factor-I receptor in human bone marrow derived mesenchymal stem cells may be used for clinical stem cell therapy in acute liver failure.