IN VITRO PLASMID CURING ACTIVITY OF AQUEOUS EXTRACT OF TERMINALIA CHEBULA FRUIT AGAINST PLASMIDS OF BACILLUS SUBTILIS AND SHIGELLA SONNEI

摘要

Objective : The aim of this investigation was to evaluate in vitro plasmid curing activity of the aqueous extract from Terminalia chebula fruit against multi-drug resistance bacteria. Methods : The aqueous extract of Terminalia chebula fruit was prepared in citrate phosphate buffer, pH 6.8. The extract was partially purified through gel chromatography. Antibacterial and antiplasmid activity against Salmonella typhi, Bacillus subtilis, Escherichia coli and Shigella sonnei was checked using the broth dilution method. The contents of crude extract were estimated. Results : The crude extracts exhibited anti plasmid activity against plasmids of B. subtilis and Sh. sonnei . Loss of plasmid band was confirmed by agarose gel electrophoresis. The curing efficiency of aqueous extract was found to be 100% and 44% against plasmids of B. subtilis (pUB110) and Sh. sonnei (pARI-815) respectively. The results were compared with conventional curing agent such as ethidium bromide and acridine. Conclusion : The aqueous extract of T. chebula fruit has the potential to inhibit growth of strains which are resistant to many antibiotics. Results could provide new insight into the treatment of infectious diseases which are facing limitation of antibiotics. Keywords: Terminalia chebula fruit , Aqueous extract, Antiplasmid activity, Antibiotic resistance.