UV-VIS SPECTROPHOTOMETRIC STUDY FOR DETERMINATION OF CEFIXIME IN PURE FORM AND IN PHARMACEUTICALS THROUGH COMPLEXATION WITH Cu(II) USING ACETATE–NaOH BUFFERIN WATER:METHANOL

摘要

UV-Vis spectroscopic method for the analysis of cefixime (CEFI)in pure form and pharmaceutical formations through complexation with Cu(II) using acetate-NaOH buffer in mixture water: methanol has been developed. Optimal temperature and time for coupling were established at 25±5oC with time ranging from 0 to10 min. The formation complex, CEFI: Cu(II ) gives maximum absorbance of the yellow color occurred at λ= 410 nm and the molar absorptivity is 5.12 x 103L.mol- 1.cm-1. The reaction between CEFI and Cu(II) occurred at a stoichiometric ratio of 1:1. All reaction conditions have been optimized to obtain the complex. Under optimum conditio ns Beer's law was obeyed at concentrations ranging from 0.2267 to 22.671μg.mL-1with correlation coefficients≥0.9995 in all cases with RSD generally less than 4.0%. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 0.030μg.mL-1and 0.091μg.mL- 1, respectively. The proposed method was simple, economic, accurate and successfully applied to the determination of CEFI in pharmaceuticals with average recovery of 98.00 to 103.00%, the results obtained agree well with the contents stated on the labels