DESMODIUM GANGETICUM ROOT AQUEOUS EXTRACT MEDIATED SYNTHESIS OF NI NANOPARTICLE AND ITS BIOLOGICAL EVALUATION

摘要

Objective : A novel, eco-friendly method of Nickel nanoparticles synthesis using aqueous extract of Desmodium gangeticum root (DG) has been reported in this study. Methods : Novel approach on synthesis of Ni nanoparticles using NiCl 2 as precursor and aqueous extract of Desmodium gangeticum root as the reducing agent. Nanoparticles were characterized for its average size, morphology, functional moieties and thermal stability by UV-Visible spectrophotometry, X-Ray Diffractometry (XRD), Scanning Electron Microscopy (SEM), Fourier Transform Infrared spectroscopy (FTIR) and Thermo Gravimetric Analysis (TGA) respectively. Cytotoxicity was also determined against LLC PK1 cell lines using LDH assay. Results : Less aggregate spherical shaped and mono-dispersed nanoparticles were synthesised whose size ranges from 20-30 nm in diameter. Nanoparticles were exhibit face centre cubic crystalline phase with an average size of ~23 nm which was obtained from XRD spectral pattern. Strong interaction between Desmodium gangeticum and nanoparticles was shown in TGA-thermogram. The reducing potential and total phenolic content of Ni nanoparticles was found to be same as that of Desmodium gangeticum . All the results were expressed as mean±SD of n = 4-6 independent assays, p<0.05, whose data were analysed using ANOVA. Conclusion : Biological activity of the nanoparticles and its?toxicity was?assessed?and found to possess the good antioxidant and reduction potential with significant antibacterial activity and were nontoxic. Keywords : Green synthesis , Desmodium gangeticum , Ni Nanoparticles, Antimicrobial, DPPH, LLC PK1.