ANTI-NOCICEPTIVE ACTIVITY OF METHANOLIC EXTRACT OF OCIMUM GRATISSIMUM (LABIATE) ON EXPERIMENTAL ANIMALS

摘要

The.methanolic.leaves.extract.of. Ocimum.gratissimum.was.investigated.for.anti‐nociceptive.effects.in.mice..The.models.used.to.study.the.effect.on.nociception.are.the.acetic.acid.induced.abdominal.constriction.test,.hot‐.plate.method.in.mice..The.extract.demonstrated.a.significant.(P<.0.05).anti‐nociceptive.activity.at.all.the.doses.(50,100.and.200.mg/kg.body.weight.i.p.).tested.group.and.standard.group.(piroxicam.20mg/kg).compared.to.control.normal.saline..The.activity.resides.more.at.the.highest.dose.200mg/kg.body.weight.i.p..that.was.found.to.have.the.highest.percentage.(92.5.%).of.inhibition.of.the.abdominal.constriction.induced.by.aceti c.acid.in.mice..Also.the.extract.was.found.to.have.a.significant.(p.<0.05).inhibitory.effect.on.hot‐plate.method.at.all.the.three.doses..The.extract.caused.a.significant.(p<0.05),.dose.dependent.inhibition.of.acetic.acid‐induced.writhi ng.and.hot‐plate.method..The.intraperitoneal.LD50.value.of.the.extract.was.1285.5mg/kg.body.weight.in.mice..Preliminary.phytochemical.screening.revealed.the.presence.of.alkaloids,.saponins,.tannins.and.flavonoids..The.results.suggest.the.extract.contained.pharmacologically.active.principles,.and.supports.the.local.application.of.the.plant.in.painful.conditions..Further.studies.may.reveal.the.exact.mechanisms.of.action.responsible.for.the.analgesic.activities.of.O..gratissimum.leaves.extract