α- GLUCOSIDASE AND ALDOSE REDUCTASE INHIBITORY ACTIVITY IN VITRO AND ANTI-DIABETIC ACTIVITY IN VIVO OF TRIBULUS TERRESTRIS L. (DUNAL)

摘要

Background:.Inhibition.of.α‐glucosidase.and.aldose.reductase.inhibitors.plays.a.major.role.in.hyperglycemia.induced.vascular.damage...It.has.also.been.implicated.as.a.major.process.affecting.the.glucose.uptake.mechanism...Objective:.Reported.study.was.done.for.a.comparative.evaluation.of.α‐glucosidase.and.aldose.reductase.inhibitory.activity.of. Tribulus.terrestris.and.Curculigo.orchioides.which.are.well‐known.Ayurvedic.Rasayana.herbs..Design:.A.total.of.48.Wistar.strain.rats.were.rendered.hyperglycemic.by.treating.them.with.streptozotocin.(75.mg/Kg.body.weight)..Diabetic.rats.were.treated.for.30.days.with.100.mg/kg.body.weight.of.ethanolic.extract.of.T..terrestris.(TT100).and.C..orchioides.(CO100).once.daily...Settings:.Enzymatic.and.other.parameters.viz.. blood. glucose. level,.glycated.hemoglobin,. insulin,.liver. glycogen.and.urinary.albumin. levels. were.measured. using. an. auto. analyzer.. Body. weight. of. the. rats. was. also.recorded.past.treatment.for.30.days.. In. vitro. α‐glucosidase. inhibition. was.determined.using.acarbose.and.epicatechin.as.controls...Main.outcome. measures:.Both.TT.and.CO.were.effective.in.inhibiting.α‐.glucosidase.and.aldose.reductase..There.was.a.reduction.in.fasting.blood.glucose.level.observed.as.well..Treatment.with.extracts.could.also.ameliorate.the.loss.of.body.weight.as.observed.in.case.of.hyperglycemic.control.group.animals..ResultS.and.conclusion:.A.significant.(p.<.0.05).decrease.in.fasting.blood.glucose.level.was.observed.in.case.of.TT100.treated.group.similar.to.the.one.treated.with.gibencalmide..A.59.6.±.2.1.%.inhibition.of.α‐.glucosidase.and.98.6%.±.1.1.%.inhibition.of.aldose.reductase.was.observed.at.a.dose.of.30.μg.in.case.of.TT100..For.CO100.the.decrease.in.fasting.blood. glucose.level. was. also.significant.(. p.<.0.05).. A. 60.4. ±. 1.2.%.inhibition. was.observed.in.case.of.α‐.glucosidase.and.99.2.±.1.4%,.inhibition.was.observed.in.case.of.aldose.reductase.activity...Elevation.in.glycated.hemoglobin.and.liver.glycogen.levels.were.also.observed.in.case.of.diabetic.rats,.and.were.similar.to.untreated.control.group.animals.in.case.of.WG100.treated.group..Similar.to.acarbose.a.significantly. higher. α‐glucosidase..inhibitory.action.was.observed. with.WG..The. low.albumin.excretion.rate.correlated.well.with.the.protective.effects.of.the.extracts..The.study.provides. molecular. interventions. for. the. utilization. of. C.. orchioides. and. T.. terrestris. in.Ayurvedic.system.of.medicine...