DETERMINATION AND COMPARISON OF THE CURCUMINOID PIGMENTS IN TURMERIC GENOTYPES (CURCUMA DOMESTICA VAL) BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

摘要

Medicinal.plants.have.the.rich.source.of.medicinally.and.agriculturally.useful.compounds.since.they.produce.raw.material.for.pharmaceutical.and.phytochemicals.for.manufacturing.drugs..Thus,.the.germplasm.collection,.conservation,.evaluation.and.cataloging.is.necessary..In.addition.to.this,.an.understanding.and.exploitation.of.their.existing.variability.is. necessary. to.find. superior. genotype. having. high. yield,. better. quality. and. high.metabolic. contents.. Considering. these. facts,. the. present. investigation. was. carried. out. on. the. important. medicinal. plant:. Turmeric.. Turmeric.( Curcuma).is.the.common.name.used.for.the.dried.rhizome.of.Curcuma.longa.L.,.a.monocotyledonous.plant.belonging.to.the.family.Zingiberaceae..It.has. been.used. in.traditional.medicine.as.a. household.remedy.for.various.diseases.including.anorexia,. cough,.diabetes,.wounds,. hepatic.disorders,.rheumatism. and. sinusitis.. Curcumin. (diferuloymethane),. the. main. yellow. bioactive. component. of. Curcuma. has. been. shown. to. have. a. wide.spectrum. of. biological. actions..These. include.its. anti‐inflammatory,. antioxidant,. ant. carcinogenic,.ant. mutagenic,.anticoagulant,. ant.fertility,. ant.diabetic,.antibacterial,.antifungal.activities..Twenty‐two.genotypes.of. Curcuma.were.collected.from.different.geographical.locations.in.Chhatisgarh.and. adjoining. areas. and. High. performance. liquid. chromatography. approached. to. determine. curcuminoids. in. different. genotypes. of. Curcuma..Curcuminoids. were. extracted. by. Soxhlet. Apparatus. with. organic. solvents. like. Ether,. Benzene. and. Ethanol. and. purified. through. column.chromatography..These.purified.fractions.were.identified.by.thin.layer.chromatography.and.subjected.to.HPLC..The.total.curcuminoid.percentage.range d. from.0.069. to.6.16.The.maximum. curcuminoid.conte nt.wa s. found.in.genotype.T22.(Wild.local.2).(6.1%).followed.by.T21.(Wild.local. 1).(4.04%),.T1.(TCP‐1).(2.817%),.T2.(TCP‐2).(2.28%)..The.variation .was.recorded.for.curcumin.content.and.its.analogs.across.all.the.genotypes..The.curcumin. content. varied. from. 1.87%. to. 0.015%.. The. highest. curcumin. percentage. was. recorded. in. the. genotype. T22. (Wild. local. 2). (1.87%).followed.by.T21.(Wild.local.1).(1.76%),.T2.(TCP‐2').(0.932%),.T15.(Maharashtra.local).(0.868%)...