RP-HPLC METHOD FOR THE ESTIMATION OF TAMSULOSIN IN BULK AND CAPSULES

摘要

Objective: To develop a simple, sensitive and precise HPLC method for the quantitative estimation of tamsulosin in capsules. Method: Chromatographic separation of the drug was achieved with an Hypersil BDS C18 column (250 mm x 4.6 mm I.D.; particle size 5 .m) analytical column using sodium percholate buffer (pH 3.0)-acetonitrile (60:40 v/v) as mobile phase. The instrumental settings are flow rate of 1.0 ml/min, column temperature at 40oC, detector wavelength of 225 nm and the run time was 10 min. Results: The retention time of the drug was 6.52 min. Theoretical plates, plates per meter and height equivalent to theoretical plate for tamsulosin were 2760.03, 11040.12 and 9.058×10-5 respectively. The developed method shows linearity over a range of 2-10 μg/ml of tamsulosin with correlation coefficient of 0.9997. The relative standard deviation is less than 2%. Conclusion: The proposed method was found to be suitable and accurate for quantitative determination of tamsulosin in capsules