DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF MESALAMINE IN BULK AND TABLET FORMULATION

摘要

A. simple. and. cost. effective. spectrophotometric. method. is. described.for.the.determination.of.mesalamine.in.pure.form.and.in.pharmaceutical.formulations..When.the.drug.reacts.with.0.5N.HCl.shows.absorption.maximum.at.303.nm.and.obeys.beer's.law.in.the.concentration.range.10‐50μg.mL‐1..The.absorbance.was.found.to.increase.linearly.with.increasing.concentration. of.MSZ,.which. is. corroborated. by. the.calculated. correlation.coefficient.value.of.0.999.(n=6)..The.apparent.molar.absorptivity.and.sandell.sensitivity.were.1.63.X.101 0and.0.0402.μg.cm‐2.respectively..The.slope.and.intercept.of.the.equation.of.the.regression.line.are.0.025. and.0.002.respectively..The.limit.of.detection.and.limit.of.quantification.was.found.to.be.0.04914.μg.mL‐1.&.0.14893.μg.mL‐1..The.validity.of.the.described.procedure.was.assessed..Statistical.analysis.of.the.result.has.been.carried.out.revealing. high. accuracy. and. good. precision.. The. proposed. method.was.successfully.applied.to.the.determination.of.MSZ.in.pharmace utica l.formulations.without.any.interference.from.common.excipients..The. relative. standard. deviations. were. ≤0.6536%,. with. recoveries. of. 98.65%. ‐101.05