COMPARATIVE STUDIES ON CISPLATIN AND ETOPOSIDE TREATED LIVER AND KIDNEY PROTEIN EXTRACT OF RAT UNDER SDS -PAGE GEL SEPARATION

摘要

Objective : Our previous studies we have documented that cisplatin dose at 0.4 mg/kg i.p for 8 weeks caused alterations at both histopathological and biochemical level however etoposide dose at 1mg/kg/ i.p for 8 weeks did not cause any hepatotoxic or nephrotoxic effect. These studies aims further the changes in protein pattern of liver and kidney tissue after drug treatment which support previous findings of any free radical induced oxidative stress on tissues. Methods : Sodium dodecyl sulphate and polyacrylamide gel electrophoresis (SDS-PAGE) of liver and kidney protein extract was separated on 5% Stacking gel and 12% Resolving gel and stained by using coomassie brilliant blue R -250. Results: Hepatic protein patteren of cisplatin treated rats showed molecular weight 44.46, 13.8, 51.2 kd and other ranging from 16.98-38.9 kd did not show any expression as compared to controls. Inaddition etoposide treatment studies showed molecular weight 14.45-33.11 kd and other ranging from 25.12 to 51.29 kd showed similar pattern of expression compared to controls. Renal protein patteren of Cisplatin treated rats showed molecular weight 36kd, 29kd, 24kd and 20kd and other in range of 66kd and 45kd did not as compared to controls. Additionally, etoposide studies depicted increase in protein level ranging from molecular weight 66kd, 45kd, 36kd, 29kd, 24kd but 20kd did not increase. Conclusion: Our studies suggest that these changes in protein levels might have significant role in organ protection