DEVELOPMENT AND VALIDATION OF METHOD FOR SIMULTANEOUS ESTIMATION OF PYRIDOXINE HYDROCHLORIDE AND DOXYLAMINE SUCCINATE IN TABLET DOSAGE FORM BY FIRST ORDER DERIVATIVE SPECTROSCOPY

摘要

Pyridoxine Hydrochloride and Doxylamine succinate are used in combination for treatment of Morning Sickness. Objective: To develop a simple spectrophotometric method for simultaneous estimation of Pyridoxine Hydrochloride (PYR) and Doxylamine succinate (DOX) in tablet dosage form. Metho d: The method employed is first order derivative spectroscopy. Results: For determination of sampling wavelength 10 μg/ml of each of PYR and DOX were scanned in 200-400 nm range and sampling wavelengths were 231.8 nm for PYR where DOX showed zero crossing point and 253 nm for DOX where PYR showed zero crossing point in first order derivative spectroscopy. For this method linearity observed in 1-40 μg/ml for PYR and 2.5-80 μg/ml for DOX. LOD of DOX and PYR were 0.2826 and 0.3571 μg/ml respectively. LOQ of DOX and PYR were 0.4621 and 0.5918 μg/ml respectively. Discussion: The recovery studies confirmed accuracy of proposed method and low values of standard deviation confirmed precision of method. The method is validated as per ICH guidelines