Molecular approach and rapid detection of virulence factor in Helicobacter pylori infection and its eradication strategies by Plumbagin

摘要

Helicobacter pylori is one of the most common pathogens affecting humankind, infecting approximately 50% of the world's population.Of those infected, many will develop asymptomatic gastritis, but 10% develop gastric or duodenal ulcers. The CagA gene is a novel marker for pathogenicity and associated with more severe clinical outcomes and PCR could be rapid and reliable technique to confirm the virulence gene in H.pylori infection. A novel rapid PCR assay was developed for accurate detection of H.pylori infection directly from patient's saliva sample which was subjected for isolation of genomic DNA that could be used as template for amplification of CagA gene fragments as PCR product of 400bp amplicon. Identification of presence or absence of cagA gene can be detected in a single reaction, directly from gastric patient's samples without need for culture. Plumbago zeylanica root extract were screened for their inhibitory effects on the strains H.pylori using the agar diffusion method and showed Plumbagin inhibits the H.pylori growth. The minimum inhibitory concentration (MIC) of the potent extracts and minimum bactericidal activity was also observed. The plasmid of H.pylori was also isolated and it was treated with the extracts showed the shearing pattern of the plasmid showed its effect against the extracts. H. pylori are found in the infected host may be efficiently eradicated by plant based drug such as plumbagin had revealed sheared plasmid DNA of the strains of H. pylori used in this study was CagA positive could be a virulence factor in H.pylori infectio