Spectrophotometric Determination of Methyl Paraben in Pure and Pharmaceutical Oral Solution

摘要

A simple, cheap and sensitive spectrophotometric method for the determination of methyl paraben in pure and dosage form has been described. The Method is based on the diazotization of the drug by sodium nitrite in acidic medium at 5Co followed by coupling with ortho-aminobenzoic acid to form orange color the product was stabilized and measured at 442 nm Beer’s law is obeyed in the concentration range of 1-9 μg?ml-1 with molar absorptivity of 1.6x106 L?mole-1?cm-1., Sandell’s sensitivity were 0.0095 μg?cm-1, The detection limit were 0.0065 μg?ml-1, and The limit of Quantitation were 0.02 μg?ml-1. All variables including the reagent concentration, reaction time, color stability period, and mole ratio were studied in order to optimize the reaction conditions. No interferences were observed Results of analysis were validated statistically and by recovery studies. These methods are successfully employed for the determination of methyl paraben in some oral solution. The developed method is easy to use and accurate for routine studies relative to HPLC and other techniques.