Study On Callus Initiation And Plantlet Regeneration Ability Of Some Rice Genotypes

摘要

Abstract: An experiment was conducted with mature embryos of three HYD and resistant rice (varieties BRRIdhan-29, BR-14, BINA dhan-8) as explants to investigate the appropriate growth condition for callus induction as well as plant regeneration on MS basal medium supplemented with different concentration of auxin and cytokinin. Different concentrations of 2, 4-D (0.0, 1.5, 2, 2.5, 3 mgL-1) were used for callus induction. Among the concentrations 2.0mgL-1 2, 4-D was best concentrations for callus induction (85%), callus size (4.533mm) and callus weight (81.847mg) in 10.66days and the highest maintaining ability was found in BR-14 at 2.0mgL-1 2, 4-D (88%) in callus size (5.143a) and callus weight (87.50mg)) in 6.50days. Different concentrations of kinetin (0.0, 0.4, 0.6, 0.8, 10 mgL-1) with a constant concentration of NAA (0.5mgL-1) were used for plant regeneration from callus. Again, BRRI dhan-29 on T3 (MS + 8 mgL-' Kn + 0.5 mgL-' NAA) was found to be best in shoot length (6.00cm) with highest shoots initiation (97.5%) and the highest .number of shoots per callus (5.25) on T2 (MS + 6 mgL-' Kn + 0.5 mgL-' NAA) in 22.25days. In combination, BRRI dhan-29 the highest number of roots per explant (7.25), root length (2.31cm) was found in T3 (MS + 0.6 mgL-1 IBA) and the highest root initiation (97.50%) was on T2 (MS + 0.5 mgL-1 IBA).