Synthesis, Characterization and Effect of the Antibacterial Activity of Chitosan Nanoparticles on Vancomycin-Resistant Enterococcus and Other Gram Negative or Gram Positive Bacteria

摘要

Chitosan, a non-toxic and biocompatible natural polymer has been used as an absorption enhancer for poorly absorbable drugs. Biodegradable polymers such as chitosan have been used extensively in biomedical fields in the form of sutures, wound covering and as artificial skin and antibiotic delivery for infection disease. The aims of research is Synthesis, characterization and effect of the antibacterial activity of chitosan nanoparticles on Vancomycin-Resistant Enterococcus and other gram negative or gram positive Bacteria. Chitosan was synthesized. Then chitosan nanoparticle is characterized. Size and zeta potential of nanoparticle was determined by DLS and SEM microscope method. The antibacterial activity of chitosan nanoparticle derivatives prepared on Vancomycin- Resistant Enterococcus, Staphylococcus aureus and Escherichia coli was examined by well diffusion and micro titer method according CLSI. Size and zeta potential of nanoparticle were 210 nm and + 11 mv by DLS. Chitosan nanoparticle has antibacterial activity on bacteria. According to well diffusion test, the highest inhibition zone was recorded against Staphylococcus aureus, lower diameter of inhibition zone was showed against Enterococcus faecalis and Escherichia coli, respectively. MIC and MBC results were showed the highest inhibitory of chitosan nanoparticles against Staphylococcus aureus. This nanoparticle can be used antibacterial agent. It can affect synergism with antibiotics. Chitosan nanoparticle will apply to pharmacology for drug delivery and caring.