To study the antioxidant capacity of Astragalus polysaccharides (APS) with different molecular weights, we used hydrogen peroxide to degrade original Astragalus polysaccharide (APS0) with an initial molecular weight of 11.03 kDa and obtained three degraded polysaccharides with molecular weights of 8.38 (APS1), 4.72 (APS2), and 2.60 kDa (APS3). The structures of these polysaccharides were characterized by 1H NMR, 13C NMR, FT-IR, and GC/MS. The degradation process did not cause significant changes in the main chain structure of APS. The monosaccharide component of APS before and after degradation was slightly changed. The antioxidant ability in vitro (removing hydroxyl and ABTS radicals and reducing ability) and in cells (superoxide dismutase and malondialdehyde generation) of these polysaccharides is closely related to their molecular weight. If the molecular weight of APS is very high or low, it is not conducive to their activity. Only APS2 with moderate molecular weight showed the greatest antioxidant activity and ability to repair human kidney epithelial (HK-2) cells. Therefore, APS2 can be used as a potential antistone polysaccharide drug.
展开▼
机译：为了研究不同分子量的黄芪多糖（APS）的抗氧化能力，我们使用过氧化氢降解了初始分子量为11.03 kDa的原始黄芪多糖（APS0），并获得了三种分子量为8.38（APS1）的降解多糖， 4.72（APS2）和2.60kDa（APS3）。这些多糖的结构通过1 H NMR，13 C NMR，FT-IR和GC / MS进行表征。降解过程并未引起APS主链结构的重大变化。降解前后APS的单糖成分略有变化。这些多糖在体外的抗氧化能力（除去羟基和ABTS自由基并具有还原能力）以及在细胞中的抗氧化能力（超氧化物歧化酶和丙二醛生成）与其分子量密切相关。如果APS的分子量很高或很低，则不利于它们的活性。只有具有中等分子量的APS2表现出最大的抗氧化活性和修复人肾上皮（HK-2）细胞的能力。因此，APS2可用作潜在的抗结石多糖药物。
展开▼
