Inhibitory Effect of Grape Seed Polyphenol Extract and Vitamin C on Melanogenesis in Cultured B16-F1 Melanoma Cells

摘要

Background and Objective: The grape seed polyphenol (GSP) is rich in flavonoids and tannins which contributes to diverse pharmacological activities. The current study was undertaken to assess the anti-melanogenesis effect of GSPE on UVA-exposed B16F1 melanoma cells by checking morphological and biochemical changes. Materials and Methods: The present study was sub-divided into three phases. In phase I, the anti-melanogenesis effect of crude grape seed polyphenol extract (GSPE) was checked by comparing with three commercial grape products (A, B and C) in non-UVA exposed B16F1 cells. During phase II, the anti-melanogenesis effect of GSPE was determined on UV-exposed B16F1 cells to reveal the effective dosage. Finally, in phase III the anti-melanogenesis effect of different experimental samples (GSPE; 50 μg, Vit C; 1 μg and combo of GSPE- Vit C; 50:1μg) were examined on UV-exposed B16F1 cells. Results: The crude GSPE at the dose of 50 μg exhibited better (pConclusion: In this study, crude GSPE (50 μg) showed the maximum melanin inhibitory activity as it successfully down regulated the tyrosinase activity.