In Vitro Culture of Jatropha Curcas L: A Biofuel Plant

摘要

In vitro study revealed that leaf disc was the best explants for micropropagation through direct induction of adventitious shoots when cultured on Murashige and Skoog's (MS) medium supplemented with thidiazuron (TDZ) (2.27 |ìM), 6-benzylaminopurine (BA) (2.22 |ìM) and indole-3-butyric acid (IBA) (0.49 |ìM). The presence of TDZ in the induction medium has greater influence on the induction of adventitious shoot buds, whereas BA in the absence of TDZ promoted callus induction rather than shoot buds. Induced shoot buds were multiplied and elongated into shoots following transfer to the MS medium supplemented with BA (4.44 |ìM), kinetin (Kn) (2.33 |ìM), indole-3-acetic acid (IAA) (1.43 |ìM) and gibberellic acid (GA3) (0.72 |ìM). Well-developed shoots were rooted on half strength MS medium supplemented with IBA (0.5 |ìM) after 30 days. Regenerated plants after two months of acclimatization were successfully.