Protective Effect of Flowerbuds of Lonicera Japonica Extract on Diabetes Mellitus Type 2 and Associated Vascular Complications in STZ-HFD Treated Rats

摘要

Objective : To study the protective effect of Lonicera Japonica Alcoholic Extract (LJALE) on Diabetes Mellitus II (DM) and associated vascular complications. Methods : Induction of Diabetes Mellitus II with 35 mg / kg STZ-HFD leads to excessive level of 11β-HSD1 enzyme. It has been found that flower buds of Lonicera Japonica (LJ) has also been traditionally indicated for treatment of diabetes. Chlorogenic Acid (CA) is a major bioactive component in the flower buds of LJ has received more attention because of its 11β-HSD1 inhibitory action.11β-HSD1 regulates glucocorticoid action at the pre-receptor stage by converting cortisone to cortisol. Elevated glucocorticoids are a key risk factor for metabolic diseases. LJALE was prepared with help of Microwave Assisted Extraction (MAE) method. STZ-HFD-induced diabetic rats were treated with LJALE (200 mg/kg, 500 mg/kg) and Metformin (50 mg/kg). It was given for 4 weeks. Result: LJALE treated Diabetic rats showed significant decreased in blood glucose levels, plasma insulin, serum cortisol as well significant increased hepatic glycogen, 11β-HSD1 index compare to diabetic control rats. LJALE (500 mg/kg) treatment showed the return of islets close to good vascular pattern. LJALE treatment restored endothelium dysfunction. Conclusions: From all results it is reasonable to conclude that LJALE (500 mg/kg) may be used in treatment of diabetes and diabetes associated vascular dysfunction.