Study on Efficacy of Different Concentrations of Gymnema sylvestre Leaves Extract against Bacterial Pathogens

摘要

This study evaluates the effectiveness of different concentrations of Gymnema Sylvestre leaves extract against microorganisms. In vitro antibacterial studies were carried out with ethanolic extract of Gymnema sylvestre leaves. Antibacterial activity of different extracts were evaluated by “Paper Disc Diffusion Method” against Escherichia coli and Staphylococcus aureus at 5%, 10% and 40% concentrations and Ampicillin, Ciprofloxacin were used as standard drugs. The ethanolic leaf extract showed significant antimicrobial activity against the selected microorganisms when compared to the standard drug. At lower concentration (10%) the zone of inhibition of Gymnema sylvetre extract against Staphylococcus aureus was 1.7mm were found higher when compared with the result of the Standard, while Extracts had maximum activity against Escherichia coli at higher concentration (40%). Increasing concentration of leaves extract shows maximum zone of inhibition against E.coli (1.9mm). The zone of inhibition of Gymnema sylvetre showing better activity against Staphylococcus aureus when compared to Escherichia coli. The results of the present study suggest that leaves of Gymnema sylvestre can be used to treating infectious diseases caused by Staphylococcus aureus and Escherichia coli.