Total Phenolic Content, Reducing Potential & Free Radical Scavenging Activity of Hydroalcoholic Leaf Extracts of Psidium guajava and Persea Americana”

摘要

The polymorphic mutation C677T in the methylenetetrahydrofolate reductase (MTHFR) gene and hyperhomocysteinemia remain controversial risk factors for venous thromboembolism (VTE). The aim of the study was to determine the total homocysteine (tHcy) levels, and the prevalence of MTHFR C677T polymorphism in patients with confirmed VTE, and healthy controls; then to investigate whether hyperhomocysteinemia and C677T polymorphism were associated with a risk of VTE. The present case control study involved 146 apparently healthy participants and 121cases. The MTHFR 677T polymorphism was genotyped by a PCR-RFLP and tHcy levels were determined by Immulite 2000. In the present study, the distribution of the MTHFR 677C/T polymorphism was not statistically different among cases and controls (p=0.29), and it was quite high with a TT genotype frequency of 15.1% among controls. Furthermore, this mutation tended to have higher plasma hcy levels among cases. Based on the 95th percentile of control values, hyperhomocysteinemia was observed in eight cases and six controls (11.1% versus 5.8%). Neither T/T homozygote (OR=1.7, 95% CI=0.8-3.4, p=0.15) nor hyperhomocysteinemia (OR=1.7, 95%IC=0.6-4.9, p=0.31) was significantly associated with venous thrombosis even after adjustment for age and sex. We conclude that, there was no evidence found for an association between MTHFR C677T and hyperhomocysteinemia and the risk of venous thrombosis. It is possible that thrombosis risk is influenced by the interaction with further prothrombotic factors. We note also that there is no rationale for genotyping the MTHFR C677T variant for clinical purposes.