Antidiabetic Activity of Ethanolic Extracts of Rhyncosia beddomei and Glycosmis pentaphylla in Alloxan-induced Diabetic Rats

摘要

The objective of the study is to evaluate the anti-diabetic activity of ethanolic extract of Rhyncosia beddomei and ethanolic extract of Glycosmis pentaphylla leaves in alloxan-induced diabetes in adult male wistar albino rats. The effect of ethanolic extract of Rhyncosia beddomei (EERB) and ethanolic extract of Glycosmis pentaphylla (EEGP) on change in blood glucose level, lipid metabolism and histology of fat were examined in the study. Alloxan-induced diabetic adult male wistar albino rats were administered ethanolic extract (200 and 400 mg/Kg, p.o) of Rhyncosia beddomei (EERB) and Glycosmis pentaphylla (EEGP) or standard drug Glibenclamide 2.5 mg/kg or normal control (0.9% Saline) or diabetic control group (Alloxan-induced diabetic rats). The blood glucose levels of rats were recorded after single dose of test and standard drug administration at 0, 1, 2, 4 & 8 hrs and multi dose drug administration for 10 days in each group. Blood glucose levels were recorded at 0, 3, 7 and 10th day of test drug administration. After the last test drug administration i.e. on 11th day of study, animals were sacrificed and performed the evaluation activities. Alloxan-induced diabetes control group animals showed statistically significant increase in blood glucose levels, total cholesterol, triglycerides, LDL-C, VLDL-C and statistically significant decrease in HDL-C as compared to normal group animals. The alloxan-induced animals received EERB (200 & 400 mg/kg) and EEGP (200 & 400 mg/kg) showed decrease in glucose levels, total cholesterol, triglycerides, LDL-C, VLDL-C and statistically significant increase in HDL-C as compared to alloxan-induced diabetes control group animals along with standard group (Glibenclamide 5mg/Kg) animals. The body weight of EERB and EEGP 200 & 400 mg/kg rats showed significantly elevated. In conclusion, the results of diabetic study indicated that test extracts of Rhyncosia beddomei and Glycosmis pentaphylla have a beneficial effect on normalizing glucose level in alloxan‐induced induced diabetic rats. This suggests the efficacy of Rhyncosia beddomei and Glycosmis pentaphylla in the maintenance of glucose homeostasis and may be used as a therapeutic agent in the management of diabetes mellitus.