Protective Effect of Anti-Convulsant Nootropics on Memory Impairment Induced by Pregabalin”

摘要

Indian medicinal plants are used since ancient times to treat different diseases and ailments as these natural products exert broad- spectrum actions. The present study was justifiably planned to raise the callus of valuable medicinal plant Holarrhena anti dysenterica L. in in vitro condition with various combinations/concentrations of plant growth regulators, and to compare the antioxidant and phytochemical content difference in the in vivo plant and in vitro grown callus of Holarrhena anti dysenterica. The callus and crude extracts were used for total phenolic contents, primary metabolite detection and antioxidant activity by (DPPH and OH radical scavenging methods). Maximum callus (88.19%) was obtained on MS medium supplemented with (2, 4-D+Kinetin) at (2.0+1.5)mg/L. Significantly higher DPPH scavenging activity (85.11%) at 280μg/ml and (58.34%) at 240μg/ml in both the extracts i.e. leaf and callus in comparison to OH scavenging activity (74.11%) at 280μg/ml and (50.30%) at 240μg/ml with phenol contents (2.11mg/g) and (1.53mg/g) were observed in leaf and in vitro raised callus extract respectively. The findings indicates greater amount of phenolic compounds leads to more potent radical scavenging effect as shown by leaf extracts of Holarrhena antidysenterica and the ability to utilize tissue culture techniques towards development of desired bioactive metabolites from in vitro culture as an alternative way to avoid using endangered plants in pharmaceutical purposes.