A Fully Validated HPLC-UV method for Quantitative and Qualitative Determination of Six Adulterant Drugs in Natural Slimming Dietary Supplements”

摘要

Potentilla polyphylla, Solanum kurzii and Paedaria foetida are used as remedies against various diseases. Free radicals are one of the critical causative factors in the pathogenesis of many degenerative diseases. Phytochemical and biological studies have made it evident that medicinal plants have potentials in the prevention and treatment of those diseases. This study aimed at screening of major phytochemicals and assessing total phenolic content and antioxidant potential of P. polyphylla, S. kurzii and P. foetida to provide a scientific basis of its ethno medical uses. Phytochemicals screening was done using standard procedures. Total phenolic content was determined using Folin-Ciocalteu’s reagent. Free radical scavenging capacity was determined by DPPH and ABTS assays. P. polyphylla, S. kurzii and P. foetida displayed strong DPPH and ABTS free radical scavenging activity. Among the extracts, P. polyphylla possess the highest free radical scavenging activity with the corresponding IC50 value of 9 μg/ml (DPPH) and 48 μg/ml (ABTS) comparable to IC50 values of reference standard ascorbic acid, 6 μg/ml (DPPH) and 16.5 μg/ml (ABTS). Total phenolic content were 535, 21.33 and 25.16 mg GAE/ g extract, respectively, in P. polyphylla, S. kurzii and P. foetida. All the tested phytochemicals were tested positive, except for alkaloids, tannins and saponin in P. polyphylla, S. kurzii and P. foetida, respectively. Our studies suggest that the phenolic content may be the principal contributor of the free radical scavenging activity. It can be inferred from the results that these medicinal plants are potential for medications.