Inhibitory Activity against Shrimp Pathogens of Bacterial Isolates from Shrimp Food, Shrimp Gut, Seawater and Seaweed

摘要

The present study was developed in-vitro , to assess the inhibitory activity against shrimp pathogens of bacterial Isolates from shrimp food, shrimp gut, seawater and seaweed . Two types of strain of commercial probiotics were isolated from aquaculture feeds named as A and B. The identified strains were Bacillus subtilis , Lactobacillus plantarum , Pseudomonas fluorescens and Staphylococcus lactis. Both commercial and isolated probiotic organisms were identified and screened against shrimp pathogens like Vibrio parahaemolyticus, Aeromonas hydrophila, Vibrio cholerae and Vibrio harveyi by agar well diffusion method. Among eight organisms in the commercial probiotics vial A and B, only four organisms showed highest zone of inhibition against shrimp pathogens. The four isolated probiotic organisms showed highest zone of inhibition against shrimp pathogens. The measurement of zone of inhibition of isolated probiotics was greater than the inhibition zone of commercial probiotics. The isolated probiotic organisms were analyzed in survival of high pH. The results obtained were shown that all the isolated organisms were survived up to pH 14. The formulated probiotics is better for shrimp aquaculture practices than the commercial probiotics