Effect of Mobile Phase Variation and Purification on Chromatogram View by Using Fruits and Rose Extracts and HPLC Method

摘要

In this study extracts obtained from fruits of bluebery and cranberry and rose leaves were chromatografied by using mobile phase variation and DAD detector. The chromatograms were evaluated under different mobile phase systems such as isocratic and gradient elutions following purification procedure. The best separation conditions of pigments of anthocyanin in extracts and efficiency of purification procedure were searched by using mobile phases in the elution of isocratic and gradient modes. The chromatogram views have shown existence of anthocyanin constituents in the all fruits and rose extracts involving separation and qualitation. The effect of purification for separation and removal of pigments has been investigated.