Resistance to Soybean Mosaic Virus with High Yield on F7 Soybean Lines

摘要

Soybean mosaic virus (SMV) is one of the viruses, which can reduce the quality and the amount of soybean [Glycine max (L.) Merr.] production. Control of the disease by planting resistant varieties to SMV is considered as the best method and environmentally safe. The aim of this research was to identify resistance to SMV with high yield on F7 soybean lines. Each of 56 best F7 lines was obtained from selection of F2 to F6 lines using the modified bulk method. Every breeding line was planted together with its parents in one block. Each line was grown in a 2 m × 3 m plot at a 40 cm × 15 cm plant spacing. The trial was set in a randomized complete block design with three replications. Resistance to SMV-T isolate was analyzed by symptom observation and serological detection using Double antibody sandwich enzyme-linked immunosorbent assay (DAS ELISA). The symptoms varied from mild, mosaic, necrotic and symptomless reactions. Out of the 56 soybean lines, 54 soybean lines reacted negative. Titre of virus showed absorbance values lower than two lines were susceptible, i.e. (1) W/PI 200485-7-8; (2) GK/Mlg 3288-7-11. The highest yield (2.59 t/ha) with the lowest disease severity category achieved by lines (GK/PI 200485-7-8). There were significant differences in disease severity and seed yields between Wilis and Gepak Kuning varieties as parents with high yield potential in breeding line. The path analysis indicated that the number of pods per plant directly affected the seed yields.