Transcriptome Analysis of Interspecific Hybrid betweenBrassica napusandB. rapaReveals Heterosis for Oil Rape Improvement

摘要

The hybrid betweenBrassica napusandB. rapadisplays obvious heterosis in both growth performance and stress tolerances. A comparative transcriptome analysis forB. napus(AnAnCCgenome),B. rapa(ArArgenome), and its hybrid F1 (AnArCgenome) was carried out to reveal the possible molecular mechanisms of heterosis at the gene expression level. A total of 40,320 nonredundant unigenes were identified usingB. rapa(AA genome) andB. oleracea(CC genome) as reference genomes. A total of 6,816 differentially expressed genes (DEGs) were mapped in the A and C genomes with 4,946 DEGs displayed nonadditively by comparing the gene expression patterns among the three samples. The coexistence of nonadditive DEGs including high-parent dominance, low-parent dominance, overdominance, and underdominance was observed in the gene action modes of F1 hybrid, which were potentially related to the heterosis. The coexistence of multiple gene actions in the hybrid was observed and provided a list of candidate genes and pathways for heterosis. The expression bias of transposable element-associated genes was also observed in the hybrid compared to their parents. The present study could be helpful for the better understanding of the determination and regulation of mechanisms of heterosis to aidBrassicaimprovement.