Study on Integration of Quantitative Trait Locus based on Meta-Analysis Related to the Resistance of Rice Sheath Blight

摘要

Rice sheath blight, caused by the soil-borne pathogen Rhizoctonia solani, is one of several important diseases which cause yield loss in rice (Oryza sativa L.) production. Resistance to disease is controlled by quantitative trait loci (QTLs), including a large number of results of mapping QTLs for rice sheath blight resistance have been reported. However, due to the large confidence interval, there were fewer QTLs that have already been mapped. Unfortunately, more closely linked molecular markers that could be used in breeding. The integration of QTL maps based on meta-analysis could be used to integrate consistent QTLs. A total of 303 QTLs related to disease-resistance in rice have not been yet available due to the large confidence intervals to achieve fine QTL linkage mapping. However, integration of multiple QTL linkage maps incorporating “consistent” QTLs which common to various maps would circumvent this problem. Here, 303 rice sheath blight resistance related QTLs collected from 30 linkage-mapped rice populations were collected. The consistent QTLs were calculated by the method of meta-analysis. The set of “consistent” markers was then integrated with the simple sequence repeat (SSR) reference map Cornell 2001 by BioMercator 2.1. Moreover, a consensus map of rice QTL conferring sheath blight was constructed. A total of 30 consistent resistance-associated QTLs and their linked markers that were localized to chromosomes 1, 2, 3, 5, 6, 7, 9 and 11 chromosomes. The map offered by this research will serve as a basis foundation of rice sheath blight resistance fine mapping, map-based cloning and marker-assisted breeding for rice sheath blight resistance.