Chiral 3-dichloroacetyl Oxazolidines Induced the Activity of Enzymes in Maize for the Detoxified Herbicide

摘要

The expression of detoxifying enzyme glutathione S-transferase (GST) and antioxidant enzyme activities in maize seedlings were studied in response to three potential herbicide safeners (3-dichloroacetyl oxazolidine and its two optical isomers). The protective effect of chiral herbicide safeners on detoxifying to chloroacetanilide herbicides butachlor in maize was evaluated. All safeners increased the expression levels of herbicide detoxifying enzymes, including GST, catalase (CAT) and peroxidase (POD) to reduce chloroacetanilide herbicide phytotoxicity in maize seedlings. Our results indicate that the R-isomer of 3-(dichloroacetyl)-2,2,5-trimethyl-1,3-oxazolidine can induce glutathione (GSH) content, activity of GST, and the affinity for 1-chloro-2,4-dinitrobenzene (CDNB) substrate in maize, which can protect maize from injury by chloroacetanilide herbicide butachlor. The information of antioxidative enzymes activation was obtained to discuss the role of chiral safener in overcoming oxidative stress caused by the herbicide in maize.