Application of a Disposable Doxorubicin Sensor for Direct Determination of Clinical Drug Concentration in Patient Blood

摘要

A novel simple method for direct detection of clinical drug doxorubicin (DOX) was developed by thedisposable sensor. The MWNTs/Polt-L-lysine modified screen printed electrode (SPE) was fabricatedto monitor the DOX pharmacokinetic in clinical blood samples. The electrochemical behavior of DOXwas observed at MWNTs/Polt-L-lysine SPE by cyclic voltammetry (CV) and square wavevoltammetry (SWV). The electrochemical oxidation peak current was greatly increased because ofnanocomposites. Under optimized conditions, the oxidation peak current of DOX measured by SWVexhibited a good linear property with the increasing of the concentration in the range of 0.0025 M to0.25M. The detection limit was founded to be 1.0 nM. Furthermore, the disposable DOX SPE wassuccessfully used to monitor the clinical DOX pharmacokinetic with good results. The DOXconcentration-time curve was also obtained by the established method. The developed DOX sensorcould be successful to monitor the therapeutic drug for clinical individualized treatment.