Development of New Thin-Film Micro-sensor for Potentiometric Determination of Amiloride

摘要

A new simple, selective, rapid and precise thin-film organic membrane based micro-sensor wasdeveloped for potentiometric determination of amiloride drug. The membrane based microchips wasconstructed using (amiloride:tetraphenyl borate) ion pair as sensing material, o-nitophenyloctyl etheras plasticizer, potassium tetrakis(4-chlorophenyl) borate as anions excluder and PVC as supportingmatrix. The thin-film micro-sensor based on this composition provides linear Nernestian response with-6sensitivity of 601 mV/ concentration decade covering relatively wide concentration range of 510 - -2 -1 110 mol L of amiloride over pH range of 3-8. The suggested micro-sensor provides fast responsetime (<10 s) and good selectivity for amiloride over some tested cations. The microchip wassuccessfully applied in direct potentiometric determination of amiloride in its pharmaceuticalformulation. The average recovery was 96% with a mean standard deviation <4%.